Days after beating teams lying first and second in Southern League Division One South and West, Uxbridge slipped up away to second bottom Taunton Town.
Memories of last year's painful defeats against the likes of struggling Bracknell and Winchester as well as dropping four points against Taunton, came flooding back as Danny Lane took advantage of a 24th minute defensive mix-up to grab the only goal of the game.
Having struggled against the wind and up the slope in the first half the Reds poured forward in the second, piling on the pressure but despite coming close to an equaliser they could not make the breakthrough as they suffered their first defeat in eight league games.
Hayes & Yeading United dominated their Conference South game with Bromley but they too were undone by a single goal from the south Londoners, who have been rejuvenated by recent wins over Team Bath and Chelmsford.
Will Hendry, Josh Scott and Tom Cadmore were unable to make the most of early chances and United were chasing the game from the 22nd minute after a goal kick was flicked on and Ryan Hall found Warren McBean who turned his marker and wrong-footed Delroy Preddie, possibly due to a deflection.
Preddie prevented Ashley-Paul Robinson doubling the lead while Hendry and Scott Fitzgerald, who had the ball in the net but was flagged off-side, might have cancelled it out before the break and in the second half United laid siege to the home goal.
Scott was twice denied, Ram Marwa saw his attempt blocked and several other efforts went just wide as United slipped to their fifth defeat of the campaign.
Life after Colin Payne began with a 1-1 draw at Concord Rangers in Ryman One North as reserve team manager Mark Barnham led North-wood to a point in Essex.
Rangers had the better of the first half opportunities but Woods finished the half strongly and six minutes into the second period Sean Sonner punished hesitancy in the Concord defence, latching on to a Mark Burgess through ball and lifting his shot over the advancing Adam Rafis.
Danny Brown might have added a second before Northwood had to withstand a succession of set pieces before they broke out with the best move of the game in which Michael Gordon ghosted past several challenges before delivering an exquisite cross for Sonner, whose header hit the outside of the near post.
Sonner went close again from Danny Power's cross and eight minutes from time Bradley Hunter was allowed space to cut into the box and fire a deserved equaliser past Mitch Swain.
Danny Buckle fired a hat-trick as Harefield United twice came from behind to out-score Leverstock Green 4-2 in the second round of the Spartan South Midlands Premier Division Cup.
David Parkinson and Steve Boad put Leverstock in front with Buckle and Paul Haynes, five minutes into first half stoppage time, bringing the Hares level and Buckle struck again in the 53rd and 79th minutes to complete his game-winning treble.