A series of events will take place during the west London game in a move to tackle homophobic, biphobic and transphobic attitudes in sport .
The colourful campaign, which also includes rugby clubs in England and Wales was launched by lesbian, gay, bisexual and transgender (LGBT) charity Stonewall in 2013, and this year a film has been produced with LGBT athletes and fans.
At the Chelsea vs Tottenham Premier League game on Saturday (November 26) at Stamford Bridge, the clubs have organised a meet up between LGBT fans groups from both teams.
The Blues will also join in the campaign by opening the game with a giant Premier League Rainbow Laces flag while the referee will lace up in rainbow colours for the match.
Meanwhile, the iconic Wembley arch over Wembley Stadium will also be lit up in rainbow colours to mark the occasion on Saturday (November 26).
The Premier League, Football Association, English Football League, Premiership Rugby and Rugby Football Union will join the November campaign.
"The Premier League is all about exciting, passionate and unpredictable football that is for everyone, everywhere," Premier League executive chairman, Richard Scudamore, said.
"The Rainbow Laces campaign complements the work clubs are doing to promote inclusion and diversity in their stadiums, and across all levels of the sport.
"Our support for the campaign, and the decision to become members of TeamPride, is further recognition that the LGBT community is a vital and integral part of our community."
Ruth Hunt, Stonewall’s chief executive, said: "It’s crucial for organisations like the Premier League, the Football Association and the Rugby Premiership to show they welcome lesbian, gay, bi and trans people.
"At the moment, many LGBT people want to take part in sport, either as players or fans, but the abuse and hate from a minority of fans can make them feel unsafe, unwelcome or unable to be themselves.
"This campaign is about encouraging people to step up and say they will not stand for abuse and the support of football and rugby clubs and associations is crucial because it gives people the confidence to do that."
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