Kamo Mokotjo's dream of playing in the 2018 World Cup came to an end on Friday night as South Africa were beaten by Senegal.
The Bafana had to win to have any chance of reaching Russia but were always up against it after Diafra Sakho scored after 12 minutes.
An uphill struggle became Mount Everest when Thamsanqa Mkhize put the ball through his own net as South Africa were beaten 2-0.
It means the best chance of Brentford representation in Russia comes from the winner of the play-off contest between Denmark and the Republic of Ireland.
Andreas Bjelland is expected to start for the Danes as they look to overcome Martin O'Neill's side, with Henrik Dalsgaard ruled out with a heel injury. Lasse Vibe, Justin Shaibu and Emiliano Marcondes were not included in the squad.
Were Ireland to qualify, a good season for John Egan, who made his international debut earlier this year, could catapult him into the squad for Russia while Alan Judge would have an outside chance if he returned to the form that saw him earn his international debut in March 2016 when he returns from a broken leg.