Two people were arrested on the first weekend of the Piccadilly line Night Tube, British Transport Police (BTP) has said.
The service was not without the delays experienced by the users of its day service for weeks, as a signal failure resulted in a ten minute wait.
One incident occurred at Heathrow Terminals one to three station in the early hours of Saturday (December 17) morning, and was followed soon after by the next incident at Leicester Square.
BTP say a 24-year-old female passenger was threatened on a train near the Heathrow station between 4.05am to 4.15am.
A 26-year-old man from London was arrested on suspicion of using threatening/ abusive/ insulting words/ behaviour with intent to cause fear of/ provoke unlawful violence. He has been bailed until January 4 2017.
The Leicester Square incident saw a 24-year-old female assaulted by a woman between 4.30am to 5am.
As BTP officers attended and dealt with the incident, one of the officers was assaulted after being being spat at by a woman.
A 23-year-old woman from London was arrested on suspicion of common assault and later detained under the Mental Health Act.
The 24-hour service commenced on Friday (December 16) despite the line being beset by major delays over the past weeks.
A Transport for London spokesman said: "We are very pleased with how our first weekend of the Piccadilly line Night Tube went.
"Aside from one signal issue which caused a delay of 10 minutes we ran a good service across the line and we received a positive response from our customers."
RMT Union had urged for the service not to go ahead, claiming it to be unsafe, stating it should have been postponed.
On Tuesday (December 20) the Piccadilly line reported a "good service", after weeks of experiencing severe delays.
There will be no Night Tube on Christmas Eve, Christmas Day or Boxing Day.
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