A 24-hour Tesco store in Feltham is one of 30 round-the-clock branches across the UK due to have their hours cut.
The grocery giant said it would begin a 45-day consultation period this week with staff at the store in High Street, Feltham, and at other branches affected by the move.
It said workers impacted would be offered shifts at other times, possibly in other nearby stores.
There are 149 Tesco stores where stock will now be replenished during the day, rather than at night, some of which are also among the 24-hour shops seeing their opening hours reduced.
A Tesco spokesperson said: "Across the UK we're making some changes in a number of our stores, including the Feltham store, to help us run them more simply and deliver the best possible service for customers.
Affected stores to open from 6am to midnight
"We understand this may be disruptive for some colleagues and where there have been changes to a colleague's role we have worked with them to ensure they are fully supported."
Tesco has already reduced the opening hours at 20 of its 24-hour stores in June.
The stores affected by the latest change will in future open from 6am to midnight each day, and from 10am-4pm or 11am-5pm on Sundays.
The announcement comes just weeks after Tesco announced selected stores along the Victoria and Central lines would open around the clock on Fridays and Saturdays to tie in with the launch of the Night Tube.
