The opening ceremony and first match between England and Fiji will see thousands of spectators make their way to Twickers - the home of the English rugby.
World cup organisers have told fans to plan ahead and allow plenty of time for their journeys.
The event is predicted to have a significant impact on Friday rush-hour traffic as road closures will affect motorists travelling in west London and Surrey.
The A316, which the M3 runs onto, will be closed at the Hospital Bridge junction, while the A316 from the M3 will be for Rugby World Cup traffic and local access only.
Motorists heading for London are instead advised to use the M25 and the M4 instead of the M3.
South West Trains is also advising supporters and commuters to allow plenty of time for their journeys and pay close attention to travel advice available online, on trains and at stations.
The A316 will be closed at different times throughout the other Rugby World Cup fixtures being held at Twickenham, which are:
- Saturday 19 September, France v Italy, kick off at 8pm
- Saturday 26 September, England v Wales, kick off at 8pm
- Saturday 3 October, England v Australia, kick off at 8pm
- Saturday 10 October, Australia v Wales kick off at 4.45pm
- Saturday 17 October, 17 Pool B v Pool A quarter final, kick off at 4pm
- Sunday 18 October, Pool A v Pool B quarter final, kick off at 4pm
- Saturday 24 October, Semi-final 1, kick off at 4pm
- Sunday 25 October, Semi-final 2, kick off at 4pm