No specifically local issues were discussed in the hour-long BBC programme, aired on May 14, despite it being held at Brunel University, in Kingston Lane.
The panelists were Conservative health secretary Jeremy Hunt MP, Labour shadow education secretary Tristram Hunt MP, UKIP leader Nigel Farage MEP, Queen guitarist and campaigner Brian May and Economist editor Zanny Minton Beddoes.
They answered questions from the audience on everything from UKIP's election campaign chief calling Mr Farage a "snarling, thin-skinned, aggressive" man to electoral reform, but made no mention of even national issues with particular local significance, such as HS2 and Heathrow Airport's proposed expansion.
Lottie Jones, of Hillingdon Against HS2, said: “It was disappointing and frustrating – disappointing not to have HS2 raised and frustrating it was a missed opportunity.”
Ms Jones, who asked about HS2 on a Question Time in 2013, said she knew dozens who had applied for a seat in the audience, wanting to ask about HS2, but who did not make the cut.
She added: “It is up to the producers which questions they go with, but I've watched Question Time before when it's been from Manchester, or it's been from the south-west, or wherever, and they talk about the services that are available there, or if there have been cuts in those areas, so they do try and make it relevant to the area where they were.”
Elizabeth Bradley tweeted: "First #bbcqt after election comes from Uxbridge but without the new local MP. Boris?"
The BBC programme, chaired by journalist David Dimbleby, has been running since 1979 and is now made by the independent production company Mentorn.
A spokeswoman for the programme said there had been no conscious effort not to allow anti-HS2 campaigners into the audience.
She said: “The questions asked on Question Time are decided on the night of the recording and are not the basis for selecting audience members.”
The spokeswoman added: “The topics discussed on Question Time reflect the questions submitted by the studio audience, and we always make sure the most popular topics are included.
"We only have an hour, so we rarely get to discuss every question that’s submitted."