The former prima ballerina choreographed 400 BA volunteers to form a 15m-wide face, complete with the infamous wink and for the first time a red nose, at the airline's Heathrow engineering base.
In keeping with this year's theme 'Make Your Face Funny For Money' its 1989 iconic 'face advert' was recreated to give it a special lift for Comic Relief.
Ms Bussell said: “I was thrilled to be invited by British Airways to help recreate their famous ‘Face’ advertisement in celebration of such a fantastic cause.
"After visiting some of the Comic Relief-funded projects in Kenya recently, I have seen first-hand how all the money raised through Red Nose Day can truly transform lives.”
British Airways’ executive chairman, Keith Williams, added: “We are hugely grateful to Darcey for pulling this off as it really is a great tribute to our advertising heritage, with a Red Nose Day twist!
"Our volunteers have come from all across the airline to piece together our face and we’re thrilled that it has worked out so brilliantly.
"As well as embracing this year’s theme - ‘Make Your Face Funny For Money’ - we hope our recreation of this famous face raises smiles and awareness of this fantastic cause.”
Other stars including television personality Vernon Kay will fly into Manchester with American footballers, the London Warriors, to take on the Halton Spartons in a gladiator type dual at Manchester airport, on Red Nose Day (March 13).
And presenter Rylan Clark will also be on hand at London City airport on Red Nose Day, posing for selfies to look ‘Funny for Money’ in return for donations.
The airline has been a major partner of Comic Relief since 2010 and since then customers and colleagues have raised more than £9m through its partnership, Flying Start, and helped transform the lives of vulnerable and disadvantaged children in the UK and around the world.