Chelsea FC's chances of moving to Twickenham Stadium suffered a blow as local councillors wrote to express their 'outright objection' to the proposal.
The Premier League toppers are considering using the venue for a season while their own Stamford Bridge home is expanded, and they have approached the Rugby Football Union (RFU) about the possibility.
Hounslow Council leader Steve Curran and Isleworth councillor Ed Mayne have both said they would be open to the idea, and Brentford and Isleworth MP Mary Macleod said more details were needed.
But ward councillors in St Margaret's and North Twickenham, where the ground is sited, have now written to the RFU expressing their opposition.
Liberal Democrat councillors Alexander Ehmann, Ben Khosa and Geoff Acton wrote jointly to RFU board members including the organisation's chairman Bill Beaumont to complain that the move would 'dramatically' inconvenience local residents by increasing traffic creating the need for extra policing.
Should the RFU fail to reject the approach, they said they would take 'all measures available' to obstruct the proposal.
They also warned in the letter that the temporary move could well last longer than a year if other stadium renovations were anything to go by.
Mr Ehmann said: "While we have nothing against Chelsea FC or their fans, the public announcement of Chelsea's interest in relocating to Twickenham is brazen and unwanted by local people.
"Many nearby residents are concerned by the RFU's silence over the proposal and each day that goes by without an answer only adds to the fear that Twickenham Stadium is available to the highest bidder, regardless of the impact on residents."
Both the RFU and Chelsea FC have said while there had been an approach there had so far been 'no discussions of substance'.