Community groups who run arts and social projects at Watermans have reacted with outrage to the news that £500,000 will be axed from the popular centre.
"This will have a huge impact on lots of different groups," said Mosinder Dosanjh, of Asian women's group Trinjans.
"Last summer we ran some workshops and then performed a comedy there written by our members. Everyone really enjoyed working with them.
"I think it's a really great resource for all sorts of people and I was really shocked to hear it could close because of this," she added.
"We're getting all of our members to sign the petition."
Frank Hutson, who runs the Moon River club, is also urging people to sign the petition and show their support for the arts centre. The club offers over 250 disabled adults the chance to enjoy DJ training and a safe, friendly disco at Watermans three times a year.
Mr Hutson said: "Our club means a huge amount to people and so do lots of the other projects at the centre. Watermans has got a real community feel - it doesn't make any sense to close it. I personally think it's outrageous."
As well as offering theatre, music and arts exhibitions the facility on Brentford High Street is home to dozens of different social and community groups for all ages, which could face an uncertain future if the funding is withdrawn.
Watermans spokesman Leigh Stops said: "We had no hint that they planned to cut our funding and all the groups who rely on us were really shocked by the news."
Too find out more about how to support the centre and what is coming up visit the Watermans website