Jota is set to face Oxford after the 'imminent bid' Brentford were told to expect failed to materialise.
The Spaniard missed the 5-1 win over Aldershot with the club being informed they were expecting an offer for the wing wizard's services.
Head coach Dean Smith also left him out of the side that drew with FC Lausanne-Sport in France yesterday but was firm in his stance after the game.
He told the club's official website: "If nobody comes in with this imminent bid, which we were told about, he will be available for the Oxford United game. We have had that chat and he will be happy to do so."
Jota, who is only interested in a move to the Premier League, has been interesting Newcastle, West Ham, Everton, Swansea and Burnley over the summer, especially after his fine form since returning from his loan spell at Eibar.
Brentford are keen to see the 26-year-old sign a new deal and they still believe there is a chance of a contract being signed.
Maxime Colin missed the game with Lausanne-Sport due to a calf injury with the club taking no chances on fitness at this stage of pre-season.