Jota has been left out of the Brentford squad for the first friendly of the campaign against Aldershot amid rumours surrounding his future at the club.
The Spaniard has been linked with a move to a Premier League club and will not take any part in the game this evening.
Luke Daniels make his first appearance from the start, with Dan Bentley on the bench and he is expected to come on at half time.
Josh McEachran gets his first taste of action after suffering an injury at Sheffield Wednesday in February.
Henrik Dalsgaard will make his first Bees appearance from the bench in the second half as will captain Harlee Dean.
The expectation is that the substitutes will form Brentford's second half XI but this may change depending on injuries.
Brentford: Luke Daniels, Yoann Barbet, Josh Clarke, Brad Clayton, Maxime Colin, John Egan, Tom Field, Josh McEachran, Justin Shaibu, Zain Westbrooke, Ryan Woods.
Brentford substitutes: Dan Bentley, Henrik Dalsgaard, Harlee Dean, Emmanuel Onariase, Chris Mepham, Florian Jozefzoon, Rico Henry, Reece Cole, Nico Yennaris, Sergi Canos, Theo Archibald.