Brentford completed a league double over Queens Park Rangers for the first time since 1965 after winning by three goals to one at Griffin Park.
The Bees were the better side over the course of the 90 minutes and took a first half lead through Yoann Barbet who turned Jota's corner home via the crossbar.
There was controversy in the second half as Conor Washington's header appeared to have crossed the line with the Frenchman trying to clear but the goal wasn't given.
Almost immediately after, Joel Lynch then brought down Maxime Colin in the penalty area and Jota tucked the spot kick away with aplomb.
Lynch made up for his error two minutes later when he headed home a free kick at the near post.
Jota, though, wasn't to be denied another derby day goal as he scored a brilliant third taking his tally to four in four west London contests. It would be five were it not for a questionable offside at Craven Cottage.
The result lifts Brentford up to ninth and leaves QPR looking over their shoulder nervously at the relegation zone.
Below are five talking points to emerge from the contest.
All hail the King (again)
I'm almost tempted to go to a previous column about Jota and copy and paste the superlatives into it however I will try and find some different ones now.
The Spanish sensation probably scored the club's goal of the season against QPR when he controlled Nico Yennaris's cross-field pass and left Jake Bidwell for dust.
He gave himself time to pick his spot and slid the ball past Alex Smithies. There was nothing he could have done better in that moment. It reminded me, to an extent, of Sergi Canos' strike against Reading.
Jota is now averaging a goal every 127 minutes since his return in January. To put that into context, Lasse Vibe scores once every 165 minutes and Scott Hogan every 161 minutes.
With two games left, I expect the Dane to finish as top scorer this season but don't rule out Jota just yet.
Jumpers for goalposts
Okay, Brentford weren't as brilliant as they were against Derby. They didn't need to be against a poor QPR side.
However, what I've noticed from the squad in recent weeks, especially in home games, is a raw enjoyment of what they're doing on the pitch.
It's almost as if they're in Gunnersbury Park having a kickabout and enjoying themselves thoroughly while doing it, never mind the 11,000 plus people watching them play.
A lot of this, I think, stems from the sheer enthusiasm Sergi Canos has. If there was a candidate for happiest footballer in England, the young Spaniard would win hands down.
His joy at playing has impacted the whole squad and this has rubbed off in the stands. There are plenty of smiling faces.
Last goal-line controversy at Griffin Park?
Without the benefit of replay, my gut instinct was Brentford had a big call go their way in the second half as Conor Washington's header looked to have crossed the line.
I felt I saw the roof of the net bulge as Yoann Barbet looked to clear the striker's goal-bound header.
The defender was in the goal himself so it must have been a close call for the linesman on the New Road side.
With the technology coming into the Championship next season, the likelihood is that this incident is the last time a 'did the ball cross the line' debate will occur.
It's not the first time this has happened to QPR or Washington this season as the picture above proves.
One more win
Brentford are now on 63 points and in ninth place, two points adrift of last season's tally of 65. A victory in either of their final two games will see them better that haul.
It may not look like much but finishing a point better off is a sign of progress. While it's not the progress Brentford fans would have all liked in August to have seen it would still represent another fine season.
There is a growing feeling that next season could be special and, were the likes of Jota to stay and the expectation is they will, then the Bees are in for an exciting season.
Brentford's biggest test awaits
The Bees have had the better of things in west London over the last two seasons but that's changed with Fulham now the leading side in the Championship.
Victory for Slavisa Jokanovic's side would mean the Whites would complete a league double over Brentford as well as qualify for the play-offs; this is something Dean Smith's side must try and prevent at all costs.
When it comes to the quality of football played, these two sides are the best in the division and next Saturday's game could well be a thriller.