The 100-year old base will closing from spring next year to "make improvements and extend its life".
There is not set date or time-length for the closure but an RAF spokesman said the site will return to full flying operations when work is complete.
The spokesman said: “An investment is required in the runway at RAF Northolt to make improvements and extend its life.
“The runway will be closed from Spring 2018 in order to complete the required works.
“The Defence Infrastructure Organisation and the RAF are working closely together to ensure that disruption is kept to a minimum.”
The announcement comes in the wake of Ealing Council suggesting that RAF Northolt could be closed to make way for 20,000 new houses.
Getwestlondon previously reported that the council suggested to the Mayor of London's office that the working RAF base could be shut down to make way for housing.
Last month the air force said there are "currently no plans" to dispose of Northolt in response to the controversial proposal.
The RAF said there is no connection between the closure and the plans to expand Heathrow Airport by building a third runway.
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