The petition appealed to Mr Johnson and Prime Minister David Cameron for help to reinstate the much-loved landmark by piling pressure on the planning inspector, as well as to hold someone responsible.
However it was dealt a blow from the Mayor’s office, which responded to the online petition - Bring Back Brentford’s Iconic Lucozade Sign: Remove the Hideous Digital Advertising Board – on Tuesday (February 23) saying he could not get involved.
They said Mr Johnson has no powers or authority over advertisement boards.
A GLA public liaison officer stated: “Unfortunately, the Mayor has no statutory powers over advertisements such as this and is therefore unable to intervene in this matter.
“The Mayor’s planning powers are strictly defined by the Mayor of London Order (2008).
“The Mayor is unable to intervene in planning proposals that do not meet this criteria, which are the sole responsibility of the local planning authority.”
A response has not been received from the Prime Minister's office.
The petition started by Isleworth-man Gary Farnan has so far received more than 6,500 signatures, from people far and wide, since it was launched at the beginning of January.
However there is a long way to go to get the 100,000 signatures needed to get the government’s full attention.