A total of 600 Londoners piled into the hall for the event which gives audience members the opportunity to put forward their questions to the London Mayor and other Assembly members.
Following the last Mayor's Question Time where the Mayor admitted the city's housing crisis "cannot be solved overnight" , audience members pressed Labour's Sadiq Khan on his progress so far on "the city's biggest crisis" and more.
Knife crime, Brexit and Islamaphobia featured in the discussion, which wrapped up after two hours of grilling from the audience.
Mr Khan took to social media to praise the number of young people who booked tickets to the event and said: "Good to hear from so many Londoners at PQT tonight."
Here, getwestlondon pick out the highlights from the questions and how, after his first six months in the office, Mr Khan addressed an eagerly waiting audience.
1. Is a not for profit letting agency still being set up for Londoners?
Mr Khan assured the lady, from Harlesden , who asked the question that the plans for housing still include City Hall creating a not for profit agency, whereby the fees are kept low and their rent prices only go up with inflation, rather than at the price the landlord may set.
He said: "They aren't going to try and rip you off and then in 12 months time increase your rent to make a profit."
During the discussion, it was raised that renters in Brent suffer the most from damp housing, with 86% of tenants struggling to get their landlord to carry out works, according to Green Party's Sian Berry.
A quarter of London live in private rented accommodation and the Assembly - including assembly member Tom Copley who heads up Housing - said they are progressing with those plans.
2. Will there, if anything, be done about the air pollution underground and on London's tube network?
An unusual question which sparked people's interest, Mr Khan agreed that the air quality underground was in fact polluted, after he spent the day monitoring it.
The Mayor revealed there is a team investigating to see if "the heat underground can be turned into energy", with other technology being explored as it has been flagged up as an issue.
3. What measures will you take against islamaphobia to ensure London is a peaceful place for everyone?
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The Mayor replied: "In the last few years, hate crime has gone up. It's an issue, I'm not going to pretend it's not.
"The laws are there, but the issue is enforcement."
He admitted he wished he had reported racism directed at him and urged that the only way islamaphobia, homophobia, anti-semitism and other hate crimes could be tackled was if people were brave enough to report the incidents to the Metropolitan Police.
4. Female Genital Mutilation (FGM) is a great concern in Harrow. What is the mayor's vision to raise awareness for FGM in schools?
The Assembly members jointly agreed that FGM is a "big concern for the entire Assembly", particularly because "cultural sensitivity" meant that people were willing to tackle the issue less.
However, they urged they "cannot let girls down just because of cultural boundaries" - and outlined their programme called PANTS which works towards educating young women about their own body and FGM.
Jennette Arnold said: "PANTS (fighting FGM) is getting our young women to know it's their body and no one in the world has the right to damage it."
MP Dawn Butler is carrying out work with five boroughs, including Brent, on a pilot scheme to protect women, particularly as there have yet to be any arrests or convictions regarding FGM.
5. Will Harrow-on-the-Hill station be getting step-free access?
It was with a smile that the Mayor admitted some funding has been given to ensure certain London stations are given step free access, "although they cannot be named yet and can't say if Harrow-on-the-Hill is one of them."
However, he said campaigners in Harrow have long fought for disability access at the station and assembly member Navin Shah had lobbied on their behalf. Watch this space, west Londoners were told.
6. Will the concern over the closure of ticket offices be looked into?
An audience member raised her concern over ticket machines not allowing passengers to purchase certain tickets, and with more offices closing, commuters are facing a difficult route just to pay for their journey.
The Mayor replied that a report looking into customer satisfaction following the ticket office decision will be released soon and it is from that, they can analyse the impact and what can next be done.
7. What will be done to keep non-UK students from choosing other places to study rather than London in the wake of Brexit?
"The policy from Theresa May to include foreign students in her cap is wrong," said Mr Khan, speaking of migration.
"They contribute to our economy, it's bad for our country.
"There are eight million Londoners and one million of those are Spanish, France, Polish and so on. As long as I'm Mayor, they will always be welcome in London."
He said there are plans which the Assembly are working on with regards to "keeping and attracting talent" in the city which will be released in due course.
Did the London Assembly answer the questions sufficiently?
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